IDENTIFY THE MOST COMMONLY MUTATED GENES IN CHRONIC LYMPHOCYTIC LEUKEMIA AND THE SIGNIFICANCE ON PROGNOSIS

Introduction: The prognosis of chronic lymphocytic leukemia (CLL) varies widely individually. clinical staging systems by Rai and Binet, cytogenetics/FISH, mutational status IGHV have been commonly used as prognostic factors for CLL patients. advent molecular panels next-generation sequencing might provide additional markers to predict the outcome In this study, we evaluate gene mutation profiles well IGVH its application predicting survival outcomes among these Methods: We performed (NGS) in 196 clinically characterized samples OSU James Cancer Hospital analyzed 172 patients from same cohort. addition, clinic-pathological data include age, gender, immunophenotyping, cytogenetics/FISH. Results: 50 genes NGS panel are explored a sequencing-based prediction model. Five (TP53, NOTCH1, SF3B1, RAS including KRAS BRAF) identified most mutated order frequencies 24.1%, 14.2%, 13.4%, 11.6%, respectively. There 66.9% unmutated but has no significant correlation between (p = 0.48). However, with substantial TP53 not SF3B1 mutations. cohort is significantly correlated pathogenic mutations present four < 0.001, n 139, Cochran’s Q test). both Kaplan-Meier Cox Regression analyses reveal that (BRAF KRAS) only one favorable outcome. Keywords: (CLL), genomics, epigenomics, other -omics, risk models No conflicts interests pertinent abstract.